polyclonal anti-tfpi antibodies  (Prolytix)

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Evaluation of a new commercial assay for the measurement of tissue factor activity of extracellular vesicles isolated from human plasma

doi: 10.1016/j.rpth.2025.103007

Figure Lengend Snippet: Measurement of extracellular vesicle (EV) tissue factor (TF) activity using the CY-QUANT MV-TF (CY) and Chapel Hill (CH) assays in the presence of an anti-TF pathway inhibitor (TFPI) antibody and levels of TFPI in EVs. Plasma was prepared from the whole blood of 4 healthy donors with lipopolysaccharide (+LPS) or without lipopolysaccharide (−LPS) stimulation. EVs were isolated from plasma by centrifugation at 20,000 × g for 60 minutes at 4 °C. (A) TF-dependent activated factor X generation was measured using the CY (yellow) and CH (blue) assays in the presence of an inhibitory anti-TFPI antibody (2H8). (B) TFPI was analyzed in EV samples from −LPS and +LPS samples by Western blotting. A polyclonal rabbit anti-human TFPI antibody was used (final concentration 3 μg/mL) to detect TFPI. A goat anti-rabbit immunoglobulin G secondary antibody (Cell Signaling, Cat. number 7074) was used at a 1:5000 dilution to detect the anti-TFPI antibody. The position of TFPI and molecular weight (MW) standards is shown. (C) Bands were quantified using ImageLab software. The Wilcoxon matched-pairs signed-rank test was used to compare the 2 groups. Data are shown as mean ± SD or individual values. ∗∗ P < .01.

Article Snippet: The membranes were incubated with a rabbit polyclonal anti-TFPI antibody [ ] (final concentration 3 μg/mL in 5% bovine serum albumin in 1× Tris-buffered saline with 0.1% Tween-20) overnight at 4 °C with rocking, washed, and then incubated with a goat anti-rabbit IgG antibody conjugated to horseradish peroxidase (Cell Signaling, Cat. number 7074, 1:5000 in blocking buffer) for 1 hour at room temperature with rocking.

Techniques: Activity Assay, Clinical Proteomics, Isolation, Centrifugation, Western Blot, Concentration Assay, Molecular Weight, Software

Journal: Scientific reports

Article Title: Effect of bone marrow blood versus peripheral blood on the hemostatic balance of osteoblasts and endothelial cells.

doi: 10.1038/s41598-025-94942-x

Figure Lengend Snippet: Fig. 2. Differentiated osteoblasts demonstrated decreased coagulation parameters compared with MSCs. Immunostaining revealed a significant reduction in the expression of TF, TFPI, heparanase and TFPI-2 in osteoblast cells. Contiguous Tables show the indicated protein staining intensity in cells. Significance was determined using the Mann–Whitney U test. Representative images were visualized at × 10 magnitude, with 0.82 MDC objective lens, captured with a Nikon E995 digital camera (Nikon, Tokyo, Japan), and processed with Adobe Photoshop software (Adobe Systems, San Jose, CA, USA).

Article Snippet: Polyclonal anti-TF, polyclonal anti-TFPI antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Coagulation, Immunostaining, Expressing, Staining, MANN-WHITNEY, Software

Journal: Scientific reports

Article Title: Effect of bone marrow blood versus peripheral blood on the hemostatic balance of osteoblasts and endothelial cells.

doi: 10.1038/s41598-025-94942-x

Figure Lengend Snippet: Fig. 8. Graphical summarization. Levels of heparanase, TF, TFPI, and TFPI-2 were reduced in osteoblasts compared with mesenchymal stem cells. Level of heparanase was lower in bone marrow (BM) plasma compared with peripheral blood (PB) plasma. BM plasma attenuated heparanase procoagulant activity and level and increased proliferation in osteoblasts and HUVECs compared to PB plasma. In addition, BM plasma increased HUVECs angiogenesis assay (tube-formation) compared with PB.

Article Snippet: Polyclonal anti-TF, polyclonal anti-TFPI antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Clinical Proteomics, Activity Assay, Angiogenesis Assay